Articles

Attitudes of young adults from the UK towards organ donation and transplantation

This study examines the attitudes of young British adults towards donating their own organs and those of their family members.

Authors: Laura Coad, Noel Carter and Jonathan Ling

The impact of vascular anastomosis time on early kidney transplant outcomes

Most studies have found cold ischemic time to be an important predictor of delayed graft function in kidney transplantation. Relatively less is known about the warm time associated with vascular anastomosis an...

Authors: Karim Marzouk, Joseph Lawen, Ian Alwayn and Bryce A Kiberd

Campath, calcineurin inhibitor reduction and chronic allograft nephropathy (3C) study: background, rationale, and study protocol

Kidney transplantation is the best treatment for patients with end-stage renal failure, but uncertainty remains about the best immunosuppression strategy. Long-term graft survival has not improved substantiall...

Authors: Richard Haynes, Colin Baigent, Paul Harden, Martin Landray, Murat Akyol, Argiris Asderakis, Alex Baxter, Sunil Bhandari, Paramit Chowdhury, Marc Clancy, Jonathan Emberson, Paul Gibbs, Abdul Hammad, Will Herrington, Kathy Jayne, Gareth Jones…

Biological interactions of CYP2C19 genotypes with CYP3A4*18, CYP3A5*3, and MDR1-3435 in living donor liver transplantation recipients

Polymorphisms in CYP2C19 are related to the metabolic oxidation of drugs to varying degrees. The CYP3A4*18, CYP3A5*3, and MDR1-3435 variant alleles are very important, particularly in tacrolimus metabolism in org...

Authors: King-Wah Chiu, Tsung-Hui Hu, Toshiaki Nakano, Kuang-Den Chen, Chia-Yun Lai, Li-Wen Hsu, Hui-Peng Tseng, Ho-Ching Chiu, Yu-Fan Cheng, Shigeru Goto and Chao-Long Chen

Endomyocardial, intralymphocyte, and whole blood concentrations of ciclosporin A in heart transplant recipients

In the early phases following heart transplantation a main challenge is to reduce the impact of acute rejections. Previous studies indicate that intracellular ciclosporin A (CsA) concentration may be a sensiti...

Authors: Ida Robertsen, Pål Falck, Arne K Andreassen, Nina K Næss, Niclas Lunder, Hege Christensen, Lars Gullestad and Anders Åsberg

Blood transfusion in deceased donor kidney transplantation

Given the unpredictable timing of deceased donor organs and the need for blood transfusion, this study was carried out to determine the rate and risk factors for transfusion in order to identifying a low-risk ...

Authors: Karim Marzouk, Joseph Lawen and Bryce A Kiberd

Protocol TOP-Study (tacrolimus organ perfusion): a prospective randomized multicenter trial to reduce ischemia reperfusion injury in transplantation of marginal liver grafts with an ex vivotacrolimus perfusion

Critical organ shortage results in the utilization of extended donor criteria (EDC) liver grafts. These marginal liver grafts are prone to increased ischemia reperfusion injury (IRI) which may contribute to de...

Authors: Sebastian Pratschke, Michael Eder, Michael Heise, Silvio Nadalin, Andreas Pascher, Peter Schemmer, Marcus N Scherer, Frank Ulrich, Heiner Wolters, Karl-Walter Jauch, Dirk Wöhling and Martin K Angele

Estimating the long term impact of kidney donation on life expectancy and end stage renal disease

Long term studies of live kidney donation do not show evidence of appreciable risks to the donor. However nephrectomy reduces total glomerular filtration rates (GFR) and is associated with increased rates of p...

Authors: Bryce A Kiberd

A pilot study of reduced dose cyclosporine and corticosteroids to reduce new onset diabetes mellitus and acute rejection in kidney transplant recipients

New onset diabetes mellitus (NODM) and acute rejection (AR) are important causes of morbidity and risk factors for allograft failure after kidney transplantation.

Authors: Edward H Cole, GV Ramesh Prasad, Carl J Cardella, Joseph S Kim, Kathryn J Tinckam, Daniel C Cattran, Jeffrey R Schiff, David N Landsberg, Jeffrey S Zaltzman and John S Gill

Access to kidney transplantation: outcomes of the non-referred

There is a concern that some, especially older people, are not referred and could benefit from transplantation.

Authors: Meteb M AlBugami, Romuald Panek, Steven Soroka, Karthik Tennankore and Bryce A Kiberd

Male human papillomavirus infection post-kidney transplant: an overlooked disease

While immunosuppressive regimens improve the overall survival of renal transplant recipients, they also contribute to the long-term complications of post-transplant malignancies. Chronic immune suppression in ...

Authors: Oksana Genzer, Suzanne E El-Sayegh, Morton J Kleiner and Mario R Castellanos

Phospho-specific flow cytometry for pharmacodynamic monitoring of immunosuppressive therapy in transplantation

Organ transplant recipients frequently suffer from toxicity or from lack of efficacy of immunosuppressive drugs, which can be attributed to individual variations in drug sensitivity. This problem can be resolv...

Authors: Carla Baan, Anne Bouvy, Ramin Vafadari and Willem Weimar

PDX1-engineered embryonic stem cell-derived insulin producing cells regulate hyperglycemia in diabetic mice

Type 1 diabetes can be treated by the transplantation of cadaveric whole pancreata or isolated pancreatic islets. However, this form of treatment is hampered by the chronic shortage of cadaveric donors. Embryo...

Authors: Sudhanshu P Raikwar and Nicholas Zavazava

The influence of perfusion solution on renal graft viability assessment

Kidneys from donors after cardiac or circulatory death are exposed to extended periods of both warm ischemia and intra-arterial cooling before organ recovery. Marshall’s hypertonic citrate (HOC) and Bretschnei...

Authors: Colin H Wilson, Hugh Wyrley-Birch, Dhakshinarmoorthy Vijayanand, Anabelle Leea, Noel M Carter, Malcolm Haswell, Anne C Cunningham and David Talbot

Alternative approaches to myeloid suppressor cell therapy in transplantation: comparing regulatory macrophages to tolerogenic DCs and MDSCs

Several types of myeloid suppressor cell are currently being developed as cell-based immunosuppressive agents. Despite detailed knowledge about the molecular and cellular functions of these cell types, expert ...

Authors: Paloma Riquelme, Edward K Geissler and James A Hutchinson

Immunoregulatory properties of rapamycin-conditioned monocyte-derived dendritic cells and their role in transplantation

In efforts to minimize the chronic administration of immunosuppression (IS) drugs in transplantation and autoimmune disease, various cell-based tolerogenic therapies, including the use of regulatory or tolerog...

Authors: Camila Macedo, Hēth Turquist, Diana Metes and Angus W Thomson

Generation of myeloid-derived suppressor cells using prostaglandin E₂

Myeloid-derived suppressor cells (MDSCs) are natural immunosuppressive cells and endogenous inhibitors of the immune system. We describe a simple and clinically compatible method of generating large numbers of...

Authors: Nataša Obermajer and Pawel Kalinski

Human tolerogenic DC-10: perspectives for clinical applications

Dendritic cells (DCs) are critically involved in inducing either immunity or tolerance. During the last decades efforts have been devoted to the development of ad hoc methods to manipulate DCs in vitro to enhance...

Authors: Giada Amodio and Silvia Gregori

Cell therapy using tolerogenic dendritic cells in transplantation

Organ transplantation is the main alternative to the loss of vital organ function from various diseases. However, to avoid graft rejection, transplant patients are treated with immunosuppressive drugs that hav...

Authors: Aurélie Moreau, Emilie Varey, Laurence Bouchet-Delbos and Maria-Cristina Cuturi

Mesenchymal stem cell-educated macrophages

Mesenchymal stem cells (MSC) mediate their immunosuppressive effects via a variety of mechanisms. One of these mechanisms involves the induction of macrophages with immunomodulatory capacities. This effect of ...

Authors: Elke Eggenhofer and Martin J Hoogduijn

The ONE Study compares cell therapy products in organ transplantation: introduction to a review series on suppressive monocyte-derived cells

Authors: Edward K Geissler

Urinary proteomic shotgun approach for identification of potential acute rejection biomarkers in renal transplant recipients

Acute rejection (AR) episodes in renal transplant recipients are suspected when plasma creatinine is elevated and other potential causes out ruled. Graft biopsies are however needed for definite diagnosis. Non...

Authors: Håvard Loftheim, Karsten Midtvedt, Anders Hartmann, Anna V Reisæter, Pål Falck, Hallvard Holdaas, Trond Jenssen, Leon Reubsaet and Anders Åsberg

The pig as a model for translational research: overview of porcine animal models at Jichi Medical University

To improve the welfare of experimental animals, investigators seek to respect the 3R principle (Replacement, Reduction, and Refinement). Even when large animal studies are essential before moving to clinical t...

Authors: Eiji Kobayashi, Shuji Hishikawa, Takumi Teratani and Alan T Lefor

Treatment of bone loss in patients with chronic liver disease awaiting liver transplantation

Most of the patients awaiting liver transplantation already have osteopenia or even osteoporosis by end-stage liver disease.

Authors: Daniel Kaemmerer, Benjamin Schmidt, Gabriele Lehmann, Gunter Wolf, Utz Settmacher and Merten Hommann

A simplified subnormothermic machine perfusion system restores ischemically damaged liver grafts in a rat model of orthotopic liver transplantation

Liver donor shortages stimulate the development of strategies that incorporate damaged organs into the donor pool. Herein we present a simplified machine perfusion system without the need for oxygen carriers o...

Authors: Tim A Berendsen, Bote G Bruinsma, Jungwoo Lee, Vincent D’Andrea, Qiang Liu, Maria-Louisa Izamis, Korkut Uygun and Martin L Yarmush

Cryo-preserved porcine kidneys are feasible for teaching and training renal biopsy: “the bento kidney”

The use of patients as the primary teaching modality for learning procedures is being questioned. While there have been advancements in the technology used for performing needle biopsies in both native and tra...

Authors: Kenjiro Konno, Koichi Nakanishi, Shuji Hishikawa, Hozumi Tanaka, Norishige Yoshikawa, Yoshikazu Yasuda, Eiji Kobayashi and Alan Lefor

Differential activation of human T cells to allogeneic endothelial cells, epithelial cells and fibroblasts in vitro

In the direct pathway, T cells recognize intact donor major histocompatability complexes and allogeneic peptide on the surface of donor antigen presenting cells (APCs). Indirect allorecognition results from th...

Authors: Dmitry Samsonov, Christopher Geehan, Craig B Woda and David M Briscoe

Diagnosis and treatment of coronary artery disease in hemodialysis patients evaluated for transplant

We present a review of current strategies for the diagnosis and treatment of coronary artery disease (CAD) in patients with advanced chronic kidney disease who are on the waiting list for transplants, based on...

Authors: Jose JG De Lima, Luis Henrique W Gowdak and Flavio J de Paula

Organ preservation using a photosynthetic solution

Organs harvested from a body lapsing into circulatory deficit are exposed to low O₂/high CO₂, and reach a critical point where original functionality after transplantation is unlikely. The present study evaluates...

Authors: Ippei Yamaoka, Takeshi Kikuchi, Tomohiro Arata and Eiji Kobayashi

Open new possibilities in Transplantation Research

Authors: Edward K Geissler and Alan Jardine