Protocol TOP-Study (tacrolimus organ perfusion): a prospective randomized multicenter trial to reduce ischemia reperfusion injury in transplantation of marginal liver grafts with an ex vivotacrolimus perfusion

Table 1 Tacrolimus and ischemia reperfusion injury experimental animal studies

Author	Cold vs warm ischemia	Ischemic time	Species	TAC-application:	End points	P
				Systemic vs organ rinse
Sakr et al., 1991 [15]	Warm	45 minutes	Rat	Systemic	Survival, aminotransferases, LDH	<0.05
Kawano et al., 1995 [16]	Warm	60 minutes	Rat	Systemic	Microcirculation, aminotransferases	<0.05
Kawano et al., 1996 [17]	Cold	80 minutes	Rat	Systemic	Lipid peroxidation, aminotransferases	<0.05
Garcia-Criado et al., 1997 [18]	Warm	90 minutes	Rat	Systemic	Survival	<0.01
Garcia-Criado et al., 1997 [18]	Warm	90 minutes	Rat	Systemic	ROS, cytokines, aminotransferases, neutrophil infiltration	<0.05
Takeichi et al., 2009 [19]	Warm	50 minutes	Rat	Systemic	Aminotransferases, neutrophil activation	<0.05
Huser et al., 2009 [20]	Cold	120 minutes	Rat	Systemic	Aminotransferases, histology	0.001
Pratschke et al., 2012 [14]	Cold	24 hours	Rat	Organ rinse	Aminotransferases, glutathione metabolism	<0.05

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