Overview of the gating strategy for panel ONE 06: dendritic cell (DC) subsets. DCs and their subpopulations were identified, as previously reported [38–41]. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45+ leukocytes as shown for panel ONE 01 (Figure 2); gating of lineage (LIN; anti-CD3, anti-CD14, anti-CD19, anti-CD20, anti-CD56) negative HLA-DR+ cells, identification of LIN-HLA-DR+CD11c+ myeloid DCs (mDCs), and LIN-HLA-DR+CD11c- cells (anti-CD11c versus anti-HLA-DR). Pre-gated mDCs were used to identify CD16+, mDC1, and BDCA3+mDC subsets, and pre-gated LIN-HLA-DR+CD11c- cells were used to identify plasmacytoid DCs (CD123+BDCA2+). DC, dendritic cell; mDC, myeloid dendritic cell; LIN, lineage; WB, whole blood.