Figure 3
From: IL-13 signaling via IL-13Rα2 triggers TGF-β1-dependent allograft fibrosis
Increased collagen deposition in allogeneically transplanted grafts. (A) Representative Masson’s trichrome stainings showed increased levels of collagen (blue color) in allogeneically (FVB into DBA/1) compared to syngeneically (DBA/1 into DBA/1) transplanted hearts explanted at day 60 and day 100 after transplantation (5× and 20× magnification). (B) Analysis by Sircol assay detected significantly higher amounts of collagen in FVB hearts placed into DBA/1 recipients at day 60 (P = 0.0342) and at day 100 after transplantation (P = 0.0022) compared to the DBA/1 to DBA/1 mice and also to FVB control hearts (P = 0.0094). At least five mice per group were analyzed. *P < 0.05.