Figure 3

System for photosynthetic respiratory assistance of harvested organs. Pancreases isolated from donor rats at 3 h after cardiac arrest are placed in preservation solution containing 1.5 g/dL bovine hemoglobin. An oxygen permeable pouch containing Chlorella in 50 mL of solution (0.6 g/dL NaHCO₃) is immersed in 50 mL of preservation solution illuminated with an immersible custom-designed device comprising seven LED lights (70,000 lux) for 30 min. Oxygen generated from Chlorella photosynthesis is transferred to preservation solution via permeable film.