Phospho-specific flow cytometry for pharmacodynamic monitoring of immunosuppressive therapy in transplantation
© Baan et al.; licensee BioMed Central Ltd. 2012
Received: 30 March 2012
Accepted: 2 August 2012
Published: 16 November 2012
Organ transplant recipients frequently suffer from toxicity or from lack of efficacy of immunosuppressive drugs, which can be attributed to individual variations in drug sensitivity. This problem can be resolved by applying pharmacodynamic monitoring that focuses on measuring the biological effects of drugs. Here we discuss the new technique called phospho-specific flow cytometry to monitor the activity of intracellular immune signaling pathways at the single-cell level in whole blood samples. Through this tool the efficacy of immunosuppressive medication can be assessed, novel targets can be identified, and differences in drug sensitivity between cells and patients can be clarified.
KeywordsImmunosuppressive medication Kidney transplantation Regulatory T cells Phospho-specific flow cytometry
To prevent and to treat alloreactivity, transplant recipients are on immunosuppressive medication for life. These drugs target the immune system in a non-specific manner by affecting immune cell activation, clonal expansion and differentiation. Standard immune suppressing regimens consists of a calcineurin inhibitor (CNI, e.g., tacrolimus/cyclosporine), an inosine monophosphate dehydrogenase inhibitor (mycophenolate mofetil, MMF) and corticosteroids. The therapeutic window of CNIs is small, which places patients at risk of toxicity in case of over-dosing and rejection when under-dosed. Examples of debilitating side effects of immunosuppressive medication are infections, diabetes, nephrotoxicity and malignancies, all influencing patient and graft survival and quality of life.
Because of these debilitating side effects, there is a great need for (1) safer and more selective immunosuppressive agents and (2) better monitoring tools and parameters.
In the recent years, new pharmacologic agents have been introduced in the transplantation clinic to more specifically target the molecules of the T-cell activation cascade. Novel agents are drugs that target T-cell receptor (TCR) signaling (e.g., sotrastaurin), co-stimulation pathways (belatacept) and cytokine signaling pathways (tofacitinib). Based on the results found in experimental models, it is expected that these novel immunosuppressants are more specific than CNI.
Here we will touch briefly on the mechanisms of action of these immunosuppressants and discuss the developments in pharmacodynamic monitoring in transplantation patients.
T-cell activation and intracellular signaling pathways: targeting signal 1
After T-cell activation, several intracellular signaling pathways are used for proliferation, differentiation and death. This involves a cascade of phosphorylation and dephosphorylation of intracellular molecules by phosphatases (enzymes that remove phosphate groups from other proteins) and kinases (enzymes that modify proteins by adding phosphate groups).
Complications after organ transplantation and therapeutic options
Complications and immune responses
Identified cell type
Key signaling molecule(s) of
Ischemia reperfusion injury (IRI)
NK and NKT cells, neutrophils, macrophages
NF-AT, NF-κB, STATs,
T-cell depletion (rATG/alemtuzumab)
MAPK-p38, ERK, JNK
B- and plasma cells
NF-AT, NF-κB, AKT
IvIG, T- and B-cell depletion (rATG, alemtuzumab, rituximab), bortezomib
MAPK-p38, ERK, JNK, AP-1
Cells from the innate and adaptive immune system
T-cell depletion (rATG/alemtuzumab)
Memory T cells,
NF-AT, NF-κB, AKT, PI3K, MAPK-p38, ERK, JNK, AP-1
Anti-CD2 fusion protein (alefacept)
Depletion of memory T cells (efalizumab)
FoxP3+ regulatory T cells
Co-stimulation: targeting signal 2
The co-stimulatory signal augments T-cell activation by interactions with cell surface molecules expressed by antigen-presenting cells (APC). In this respect, augmenting interactions are: CD40/CD40ligand, CD28/CD80 and/or CD28/CD86. Inversely, T-cell activation is reduced by the co-stimulation molecule cytotoxic T-lymphocyte antigen (CTLA)-4, which transmits a T-cell inhibitory signal after binding to CD80 and/or CD86. The humanized receptor conjugate of CTLA-4 and IgG1, CTLA-4 Ig (belatacept), which indirectly blocks CD28 signaling, is approved for use in organ transplantation. Blockade of CD28 activation prevents the activation of NF-κB activation, the transcriptional target for co-stimulatory activity.
Two recently completed phase 3 trials of belatacept (nulojix®) in kidney transplantation, the so-called BENEFIT and BENEFIT-EXT studies, indicate that this agent is a safe and effective immunosuppressant leading to significantly better renal function (GFR, glomerular filtration rate) as compared to a cyclosporine-based regimen in kidney transplant recipients[8–10]. Acute rejection rates were higher but clinically acceptable among belatacept-treated patients (17-22% at 12 months in the belatacept groups versus 7-14% in the cyclosporine groups). While posttransplant lymphoproliferative disease was also more common in those on belatacept, this was largely limited to previously EBV seronegative recipients.
Cytokine signaling: targeting signal 3
The third option studied to treat organ transplant patients with more selective immunosuppressive medication is targeting signal 3: the IL-2 signaling pathway. Blockade of the IL-2 pathway prevents T-cell differentiation and production of effector molecules. This can now be achieved with the immunosuppressant named tofacitinib, formerly known as CP-690,550 and tasocitinib (Table1). The Janus kinase (JAK)/signal transducer and activator of the transcription (STAT) signal transduction pathway is essential in transmitting cytokine-mediated signals of the IL-2 family members (IL-2, IL-4, IL-7, IL-15, IL-21) to the nucleus in order to alter gene expression programs. JAKs possess two near-identical phosphate-transferring domains (, Figure1B). One domain exhibits the kinase activity, while the other negatively regulates the kinase activity of the first. The four enzymes JAK1, JAK2, JAK3 and tyrosine kinase (TYK) 2 transduce cytokine-mediated signals. After recruitment, STATs are phosphorylated by the JAKs at a specific activating tyrosine residue. This results in dissociation from the receptor, formation of STAT homo- or heterodimers, and translocation to the nucleus. Seven STATs have been identified that after activation interact with specific DNA sequences in target promoters to modify gene expression. For instance IL-2 receptor signaling is mediated through activation of JAK1 and JAK3 with subsequent phosphorylation and activation of STAT3 and STAT5, which are key molecules for T-cell development and activation.
Treatment with the JAK1/3 antagonist tofacitinib in kidney transplant recipients demonstrated acceptable safety and tolerability in combination with mycophenolate mofetil. Tofacitinib-based treatment resulted in relatively low acute rejection rates but was unfortunately accompanied by high infection rates suggestive of over-immunosuppression.
Rational for pharmacodynamic immunomonitoring
Overall the novel immunosuppressants currently being tested in phase II and III trials (sotrastaurin, tofacitinib) and the FDA-approved belatacept failed to show potent, selective immunosuppression. To gain insight into the mechanisms behind these unexpected side effects and the sometimes observed lack of efficacy, it is useful to perform immune monitoring. This will give answers to the questions about the selectivity and efficacy of these novel immunosuppressive compounds. By immune monitoring, questions like the following will be unraveled: “Do the agents indeed block their target(s) in vivo?” “How do naïve and antigen-experienced memory T cells behave?” “Do immunosuppressive agents influence the function of the suppressor regulatory T cells (Tregs) that control immune reactivity?” “Does redundancy in the cytokine network affect the efficacy of the studied immunosuppressant?” Recent developments in phospho-specific flow cytometry now provide the opportunity for routine measurements of intracellular signaling molecules in different T-cell subsets. In the next part of the article, we will discuss this tool with its recent developments and applications to better determine the balance between drug efficacy and side effects for the individual patient.
Immune monitoring by classical flow cytometry
Flow cytometry is the standard immune monitoring method to measure T-, B- and NK-cell numbers in the circulation of rATG/alemtuzumab-treated patients and to verify CD25 blockade on T cells during basiliximab therapy. Reports on these studies showed correlations between cell numbers and the occurrence of opportunistic infections and the malignancies without providing insight on the function of the repopulated T cells and of T cells covered with anti-CD25 mAb[15–18]. For instance the infections in rATG-treated patients are explained by the low T-cell and NK-cell numbers. However, there is now evidence that the function of the repopulated T cells is impaired, which may affect the immunity of rATG-treated patients[17–19]. Also the role of regulatory T cells (Tregs) in clinical organ transplantation is often studied by their phenotype. One of the first papers describing a role for these cells in drug-free tolerant kidney transplant patients was by Louis et al., who reported high numbers of peripheral Tregs compared to patients with chronic rejection. In line with effector T cells, also the numbers and function of Tregs are influenced by immunosuppressive drugs whereby both Treg-favoring and -hampering effects were reported[21–25]. Also, the first studies analyzing the mechanisms of action of tofacitinib and belatacept report an effect on peripheral Treg numbers. A significant decrease in Treg numbers with unexpected potent regulatory capacities of tofacitinib-treated patients was found. Additional information on why the suppressive function was not influenced by tofacitinib or how rATG triggers the induction of Tregs in vivo would have been helpful to understand these findings.
Accordingly, there is a clear-cut need for methods that provide information at the molecular level of immune competent cells. A method that recently became available for clinical research and diagnostics and offers these applications is phospho-specific flow cytometry. By this method intracellular signaling pathways at the single-cell level can be measured. It is a quick and reliable method that combines the extracellular cell surface characteristics with intracellular molecular activities and thus will provide the additional information required for to better understand T-cell functions during immune responses in immunosuppressed transplantation patients.
Phospho-specific flow cytometry: a novel tool to measure intracellular signaling pathways
With the recent advances in flow cytometry, the number of parameters that can be measured has been largely expanded. These new parameters now allow us to monitor immune responses functionally at rest and following activation at the single-cell level. This includes parameters such as ligand-induced activation, intracellular cytokine production, cytotoxic and proliferative activities, and most recently intracellular signaling pathways. The availability of phospho-specific antibodies makes it possible to study protein phosphorylation at the posttranslation level, thereby influencing the activity of intracellular proteins. Protocols have been developed that can be used to study, in whole blood, cellular activation cascades both in the presence and absence of agents that stimulate or inhibit cellular functions. This approach will provide information on the dynamics of the immune responses seen in organ transplant patients. But one should be aware that this is only the beginning of this new research area as more than 80,000 unique phosphorylations have been described in mammalian cells (http://www.phosphosite.org).
Challenges to clinical practice and limitations of flow cytometry
The clinical applications for phospho-protein analysis by flow cytometry are clear: the efficacy and side effects of immunosuppressive drugs, pharmodynamic profiling, cellular functions and disease activity. However, like any other laboratory technique, also this method has its typical difficulties. A limitation is the availability of antibodies of interest. Yet it is expected that more and more phospho- and epitopic-specific antibodies will become available soon. For instance, antibodies that detect TCR-induced T-cell activation would be highly valued. These antibodies can be used to determine whether or not T cells are present in the peripheral blood of organ transplant patients that respond to donor antigen. Analysis of activated Zap-70 will provide this key information and will at the same time be the functional alternative that overcomes the difficulties and limitations of TCR tetramer flow cytometry. Further, simultaneous measurements of phosphorylated proteins together with cytokines are far from optimal, and reproducibility from one experiment to the next remains a problem. In this respect, standardization of the instrument using multicolor bead samples is helpful. Data interpretation is also a question to address: should percentages or mean fluoresce intensities (MFI) be used. The relative MFI values are based on control samples like the unstained control samples, isotype control or fluorescent minus one control to set thresholds and are used to measure a shift in fluorescence intensity of the entire cell populations. Consequently, it is not clear how many cells of the entire cell population have changed expression of the molecule of interest.
As stated in the paper by Herzenberg et al., interpreting flow cytometry data is the ‘Tower of Babel’: negative, unstained populations can seem to be positive and vice versa. Therefore, optimization and standardization are key for the success of (phospho-specific) flow cytometry for measuring immune responses in healthy and diseased individuals. Addressing these limitations will further improve the utility of phospho-specific flow cytometry for clinical applications.
Recently, phospho-specific flow cytometry came forward as a new and powerful tool to analyze the activity of intracellular signaling pathways by rapid and sensitive detection of intracellular phosphorylated proteins at the single-cell level. It is a technique that can be used to study (pathological) conditions in immune cells, can be used as a screening tool to identify novel targets of immunosuppressive drugs and for pharmacodynamic monitoring of patients on immunosuppressive medication. In particular patients treated with drugs that target these intracellular signaling molecules: the inhibitors of calcineurin, mTOR and kinase activity should be monitored by phospho-specific flow cytometry.
Analysis of signaling pathways in large patient populations will show patient-specific differences in immune reactivity, drug susceptibility and drug-related side effects.
Rabbit anti thymocyte globulin
Cytotoxic T-lymphocyte antigen-4
Glomerular filtration rate
The linker for activated T cells
Lymphocyte protein-tyrosine kinase
Mitogen-activated protein kinase
Mean fluoresce intensities
Mammalian target of rapamycin
Protein kinase C
Signal transducer and activator of transcription
Regulatory T cells
Tyrosine kinase 2.
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