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Fig. 1 | Transplantation Research

Fig. 1

From: Some considerations on the current debate about typing resolution in solid organ transplantation

Fig. 1

An overview of DNA sequencing by synthesis technology (Illumina). After random fragmentation of genomic or cDNA, adapters, which are specialized oligonucleotides, are bound to both 5 and 3 DNA fragment ends, allowing ligation and NGS library preparation. The library is loaded into a flow cell that is a glass slide with one (for MiSeq technology) or eight lanes (for HiSeq, allowing eight independent experiments) coated with surface-bound, adapter-complimentary oligos. The sample is hybridized onto the flow cell, generating a cluster and is clonally amplified through bridge amplification until the cluster has 1000 copies. Each cluster on the flow cell produces a single sequence read. The flow cell is imaged, and the emission from each image is recorded. Finally, the reads are aligned to a reference sequence using bioinformatics tools

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