Figure 6

Cytokine analysis of lung tissue. qPCR analysis was performed on the lung tissue samples collected from the MAPC-treated LLL and vehicle-treated RLL of lungs 2–4 at t = 0, 2, and/or 4 h. The fold expression represents the levels of the target gene compared to the t = 0 value. All data were normalized to a housekeeping gene, GAPDH. Data represents means ± standard deviations from LLL and RLL samples from lungs 2–4. Although strong trends towards differences at 2 h were observed in several of the measured mRNA levels of mediators typically associated with MSC immunomodulation, none reached statistical significance.