Single CV values of all cell subsets tested within intra-assays (whole blood (WB) material from two healthy individuals; 71 single subsets = 142 data points). CV values include five replicates assayed in parallel. Shown are the function, regression, and 95% confidence interval of the CV versus counted events, CV versus calculated absolute cell number of the gated subpopulations, and CV versus percentage of the gated subpopulation. Also shown are the calculated lower limits of quantitation (LoQ) and the upper LoQ for a given CV. CV, coefficient of variation; LoQ, limit of quantitation; WB, whole blood.