Figure 5

Overview of the gating strategy for panel ONE 04: memory T cells and regulatory T cells. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45⁺ leukocytes as shown for panel ONE 01 (Figure 2); gating of CD3⁺ T cells (anti-CD3 versus sideward scatter); gating of CD4⁺ as well the CD8⁺ T cells (anti-CD4 versus anti-CD8), for both subsets gating of naive (CCR7⁺ or CD62L⁺ and CD45RA⁺), central memory (CCR7⁺ or CD62L⁺ and CD45RA^-), effector memory (CCR7^- or CD62L^- and CD45RA^-), and TEMRA (CCR7^- or CD62L^- and CD45RA⁺) subsets, as reported recently [33, 34]. CD4⁺CD25⁺⁺ were further separated into CD127^low regulatory T cells, discriminating CD45RA⁺ naive and CD45RA^- memory regulatory T cells, and CD127^high activated effector T cells [35]. We also enumerated activated CD8⁺CD25⁺⁺ cells. WB, whole blood.