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Figure 5 | Transplantation Research

Figure 5

From: Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study

Figure 5

Overview of the gating strategy for panel ONE 04: memory T cells and regulatory T cells. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45+ leukocytes as shown for panel ONE 01 (FigureĀ 2); gating of CD3+ T cells (anti-CD3 versus sideward scatter); gating of CD4+ as well the CD8+ T cells (anti-CD4 versus anti-CD8), for both subsets gating of naive (CCR7+ or CD62L+ and CD45RA+), central memory (CCR7+ or CD62L+ and CD45RA-), effector memory (CCR7- or CD62L- and CD45RA-), and TEMRA (CCR7- or CD62L- and CD45RA+) subsets, as reported recently [33, 34]. CD4+CD25++ were further separated into CD127low regulatory T cells, discriminating CD45RA+ naive and CD45RA- memory regulatory T cells, and CD127high activated effector T cells [35]. We also enumerated activated CD8+CD25++ cells. WB, whole blood.

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