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Figure 4 | Transplantation Research

Figure 4

From: Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study

Figure 4

Overview of the gating strategy for panel ONE 03: T cell activation. Expression of CD57 or HLA-DR and loss of CD27 or CD28 expression was used as a sign of T cell activation, as previously described [2732]. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45+ leukocytes as shown for panel ONE 01 (Figure 2); gating of CD3+ T cells (anti-CD3 versus sideward scatter); and gating of CD4+ as well the CD8+ T cells (anti-CD4 versus anti-CD8), for both subsets gating on CD57+ cells (anti-CD57 versus sideward scatter), HLA-DR+/CD45RA+ (naive, and HLA-DR+/CD45RA- (memory), and CD27-/+ and CD28-/+ subsets (anti-CD27 versus anti-CD28). WB, whole blood.

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