Figure 3

Overview of the gating strategy for panel ONE 02: T cell subsets/αβ ⁺ T cells and γδ ⁺ T cells. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45⁺ leukocytes as shown for panel ONE 01 (Figure 2); gating of CD3⁺ T cells (anti-CD3 versus sideward scatter); gating of αβ⁺ T cells and γδ⁺ T cells (anti-T cell receptor αβ⁺ T cells versus anti-T cell receptor γδ⁺); and gating of CD4⁺ and CD8⁺ T cells for both T cell receptor subsets (anti-CD4 versus anti-CD8). WB, whole blood.