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Figure 3 | Transplantation Research

Figure 3

From: Standardization of whole blood immune phenotype monitoring for clinical trials: panels and methods from the ONE study

Figure 3

Overview of the gating strategy for panel ONE 02: T cell subsets/αβ + T cells and γδ + T cells. The data file of the stained lysed (EDTA spiked) whole blood (WB) was analyzed as follows: exclusion of non-single events and gating of CD45+ leukocytes as shown for panel ONE 01 (Figure 2); gating of CD3+ T cells (anti-CD3 versus sideward scatter); gating of αβ+ T cells and γδ+ T cells (anti-T cell receptor αβ+ T cells versus anti-T cell receptor γδ+); and gating of CD4+ and CD8+ T cells for both T cell receptor subsets (anti-CD4 versus anti-CD8). WB, whole blood.

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