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Figure 1 | Transplantation Research

Figure 1

From: Differential activation of human T cells to allogeneic endothelial cells, epithelial cells and fibroblasts in vitro

Figure 1

Transfer of the dye from EC, fibroblasts or RPTEC to CD14+monocytes in transmigration assays. Confluent monolayers of EC, fibroblasts and RPTEC were grown on transwell inserts and labeled with the lipophylic dye, DiOC-16. Labeled cells were washed extensively before each experiment. A total of 1x106 PBMC were placed into the upper chamber of the transwell, were allowed to transmigrate into the lower chamber and were collected after 1.5h. Panel A, DiOC uptake was assessed by FACS analysis on CD14+ monocytes and on CD4+ or CD8+ T cells following transmigration. Panel B, Uptake of DiOC was assessed on CD14+ monocytes following interactions with EC undergoing apoptosis. Apoptosis was induced in EC by TNF- and cyclohexamide treatment. The percent double positive cells is shown in the right upper corner of each panel. Results are representative of 10 experiments for each cell type, and was identical when different PBMCs and cell lines were used

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