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Table 1 Nucleotide sequence of the PCR primers

From: PDX1-engineered embryonic stem cell-derived insulin producing cells regulate hyperglycemia in diabetic mice

Gene Forward Primer Reverse Primer
Oct4 5′GGCGTTCTCTTTGGAAAGGTGTTC3′ 5′CTCGAACCACATCCTTCTCT3′
E-cadherin 5′AAACTTGGGGACAGCAACATCAG3′ 5′TCTTTTGGTTTGCAGAGACAGGG3′
CXCR4 5′CGGGATGAAAACGTCCATTT3′ 5′ATGACCAGGATCACCAATCCA3′
Ngn3 5′TGGCACTCAGCAAACAGCGA3′ 5′ACCCAGAGCCAGACAGGTCT3′
Isl1 5′AGATATGGGAGACATGGGCGAT3′ 5′ACACAGCGGAAACACTCGATG3′
Foxa2 5′ACCTGAGTCCGAGTCTGACC3′ 5′GGCACCTTGAGAAAGCAGTC3′
Pax4 5′AAATGGCGCAGGCAAGAGAA3′ 5′ATGAGGAGGCCACAGGA3′
Pax6 5′CAGTCACAGCGGAGTGAATC3′ 5′CGCTTCAGCTGAAGTCGCAT3′
Glucagon 5′ACTCACAGGGCACATTCACC3′ 5′CCAGTTGATGAAGTCCCTGG3′
Insulin 1 5′CCAGCTATAATCAGAGACCA3′ 5′GTGTAGAAGAAGCCACGCT3′
Insulin 2 5′TCCGCTACAATCAAAAACCAT3′ 5′GCTGGGTAGTGGTGGGTCTA3′
PC2/3 5′AGAGATTCCATTGTGTGGGA3′ 5′CAAAATGGACTTGGTGCCCA3′
18S rRNA 5′TAACGAACGAGACTCTGGCAT3′ 5′CGGACATCTAAGGGCATCACAG3′
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Transplantation Research

ISSN: 2047-1440

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