Figure 1

Representative recorded perfusion traces of a single kidney. (A) Pressure during intra-arterial cooling (IAC) with histidine-tryptophan-ketoglutarate (HTK) (blue); (B) after pulsatile reperfusion with Krebs Hensleit (red) reperfusion media. The arrow represents start of supplementation with phenylephrine and a rapid rise in mean pressures to around 200 mmHg. In (C) a 200 μl vehicle bolus is given with no change in mean perfusion pressure. However, in (D) 200 μl acetycholine stimulates endothelial dependent relaxation approximately 45 s later. Y-axis, mmHg; X-axis time (s)