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Table 3 Generation of immature RAPA-DC

From: Immunoregulatory properties of rapamycin-conditioned monocyte-derived dendritic cells and their role in transplantation

  Research Clinical
Monocyte isolation CD14 microbeads (AutoMACS® or CliniMACS®) Elutriation or CD14 microbeads (CliniMACS®)
Culture conditions 10% fetal calf serum 10% human serum
(7 days) AIM-V media AIM-V media (c-GMP grade)
  1000 U/mL rhIL-4 1000 U/mL rhIL-4 (c-GMP grade)
  1000 U/mL GM-CSF 1000 U/mL GM-CSF (c-GMP grade)
  10 ng/mL Rapamycin 10 ng/mL Rapamycin (c-GMP grade)
  Cell culture plates Cell culture plates or Aastrom Replicell system