Research | Clinical | |
---|---|---|
Monocyte isolation | CD14 microbeads (AutoMACS® or CliniMACS®) | Elutriation or CD14 microbeads (CliniMACS®) |
Culture conditions | 10% fetal calf serum | 10% human serum |
(7 days) | AIM-V media | AIM-V media (c-GMP grade) |
1000 U/mL rhIL-4 | 1000 U/mL rhIL-4 (c-GMP grade) | |
1000 U/mL GM-CSF | 1000 U/mL GM-CSF (c-GMP grade) | |
10 ng/mL Rapamycin | 10 ng/mL Rapamycin (c-GMP grade) | |
Cell culture plates | Cell culture plates or Aastrom Replicell system |