Figure 1

Influence of culture medium on human dendritic cell differentiation in vitro. Four populations of DC were generated in two different culture media, either RPMI/albumin or AIMV and with two different cytokine conditions, either low-dose GM-CSF (GM-DC) or GM-CSF and IL-4 (Control DC) for 6 days. (A) The four DC populations were cultured with allogeneic T cells at different ratios for 6 days. A representative experiment is shown on the left-hand panel. On the right, the results of the ratio of one DC to four Tcells are expressed as the mean T cell proliferation + standard error of the mean for three different donors (*** P < 0.001, paired T tests). (B) The four populations were un-stimulated (grey solid histogram) or stimulated with 200 ng/mL LPS and 50 ng/mL IFN-γ (black line) for 48 hours. Cell surface expression of CD80, CD83 and CD86 was analyzed after 48 hours of culture by flow cytometry. Data shown are representative of two experiments performed.